Dicalcium phosphate dihydrate가 포함된 mineral trioxide aggregates의 세포독성 및 조직염증반응
Evaluation of cytotoxicity and inflammatory tissue response of mineral trioxide aggregates containing dicalcium phosphate dihydrate
박현제, 박희철, 손기태, 김용준, 우레레, 최상훈, 양형철,
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박현제 ( Park Hyun-Jae ) - Seoul National University School of Dentistry Department of Dental Biomaterials Science
박희철 ( Park Hee-Chul ) - Seoul National University School of Dentistry Department of Dental Biomaterials Science
손기태 ( Son Gi-Tae ) - Seoul National University School of Dentistry Department of Dental Biomaterials Science
김용준 ( Kim Yong-Joon ) - Seoul National University School of Dentistry Department of Dental Biomaterials Science
우레레 ( Wu Le-Le ) - Seoul National University School of Dentistry Department of Dental Biomaterials Science
최상훈 ( Choi Sang-Hoon ) - Seoul National University School of Dentistry Department of Dental Biomaterials Science
양형철 ( Yang Hyeong-Cheol ) - Seoul National University School of Dentistry Department of Dental Biomaterials Science
Abstract
본 연구에서는 Mineral Trioxide Aggregate(MTA)의 생체적합성에 대한 dicalcium phosphate dihydrate (DCPD)의영향을 평가하고자 하였다. MTA 경화 시 발생하는 pH 상승을 억제하고자 DCPD를 OrthoMTA에 첨가하여 세포독성과피하염증반응의 변화를 관찰하였다. 인산완충생리식염수에 침지한 OrthoMTA와 DCPD-OrthoMTA의 pH를 측정한결과, OrthoMTA와 DCPD-OrthoMTA는 측정 1일째에 각각 12.5와 12.0의 pH를 보였다. 마지막 측정시점인 19일에OrthoMTA는 평균 11.6을 보인 반면, DCPD-OrthoMTA는 8.8을 나타내었다. NIH 3T3 섬유아세포에 대한 세포독성은높은 농도의 용출액(50% 이상)에서 DCPD가 OrthoMTA의 세포독성을 억제하는 효과를 보였다(p < 0.05). 마우스 피하이식시험에서는 ProRoot MTA, OrthoMTA, DCPD-OrthoMTA 간의 염증반응의 차이는 발생하지 않았다. 따라서 DCPD의MTA 세포독성을 낮추는 효과와 본 실험 조건에서의 피하염증발생에 대한 영향은 서로 유의한 상관관계를 보이지않는 것으로 사료된다.
The purpose of this study was to evaluate the effect of dicalcium phosphate dihydrate (DCPD) on the biocompatibility of mineral trioxide aggregate (MTA). DCPD was added to MTA (OrthoMTA) to suppress the increase in pH of MTA during hardening, and the change of pH, cytotoxicity, and subcutaneous inflammation reactions in mouse model were observed. The pH of OrthoMTA and DCPD-OrthoMTA at 1st day in phosphate-buffered saline was 12.5 and 12.8, respectively. At 19th day, the pH was 11.6 (OrthoMTA) and 8.8 (DCPD-OrthoMTA). Cytotoxicity of DCPD-OrthoMTA extract was lesser than that of OrthoMTA at high concentration (above 50%) (p<0.05). No significant differences appeared in subcutaneous inflammatory reactions among ProRoot MTA, OrthoMTA and DCPD-OrthoMTA. Therefore, it is likely that there is no apparent relationship between the cytotoxicity and subcutaneous inflammation in our experimental conditions.
키워드
Mineral trioxide aggregates; Dicalcium phosphate dihydrate; 세포독성; 피하염증
Mineral trioxide aggregates; Dicalcium phosphate dihydrate; Cytotoxicity; Subcutaneous inflammation
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